X. Liu, A. Pavlovsky, and R. E. Viola, The
Structural Basis for Allosteric Inhibition of a Threonine-sensitive
Aspartokinase, J. Biol. Chem. 283, 16216-16225 (2008).
R. E. Viola, L. Yerman, J. M. Fowler, C. G.
Arvidson and R. R. Brubaker, A Missense Mutation Causes Aspartase deficiency in
Yersinia pestis, Microbiology 154, 1271-1280 (2008).
J. Le Coq, A. Pavlovsky, R. Malik, R.
Sanishvili, C. Xu, and R. E. Viola, Examination of the Mechanism of Human Brain
Aspartoacylase through the Binding of an Intermediate Analog, Biochemistry 47,
3484-3492 (2008).
R. E. Viola, X. Liu, J. F. Ohren, and C. R.
Faehnle, Structure of a redundant enzyme. The second isoform of Aspartate
β-semialdehyde dehydrogenase from Vibrio cholerae, Acta. Cryst. D64, 321-330
(2008).
X. Liu, L. Hanson, P. Langan, and R. E. Viola,
The Effect of Deuteration on Protein Structure. A High Resolution Comparison of
Hydrogenous and Perdeuterated Haloalkane Dehalogenase, Acta Cryst D63,
1000-1008 (2007)
D. M. Coe and R. E. Viola, Assessing the Roles
of Catalytic Functional Groups in the Mechanism of Homoserine
Succinyltransferase, Arch. Biochem. Biophys. 461, 211-218 (2007).
J. F. Ohren, M. L. Kundracik, C. L. Borders, P.
Edmiston, and R. E. Viola, Subunit Asymmetry and Communication in Muscle
Creatine Kinase, Acta Cryst. D63, 381-389 (2007).
R. E. Viola, The Impact of Structural Biology on
Neurobiology, Proc. Natl. Acad. Sci. USA 104, 399-400
(2007).
C. R. Faehnle, X. Liu, A. Pavlovsky, and R. E.
Viola, The Initial Step in the Archael Aspartate Biosynthetic Pathway Catalyzed
by a Monofunctional Aspartokinase, Acta Cryst. F62 ,
962-966 (2006).
C. R. Faehnle, J. Le Coq, X. Liu, and R. E.
Viola, Structural Complexes of the Anti-bacterial Target
Aspartate-β-semialdehyde Dehydrogenase from Streptococcus pneumoniae, J.
Biol. Chem. 281, 31031-31040 (2006).
J. Le Coq, H.-J. An, C. Lebrilla, and R. E. Viola,
Characterization of Aspartoacylase: the Brain Enzyme Responsible for Canavan
Disease, Biochemistry 45, 5878-5884 (2006).
Q. Liu, J. R. Kirchhoff, C. R. Faehnle, R. E.
Viola, and R. A. Hudson, A Rapid Method for the Purification of Methanol
Dehydrogenase from Methylobacterium extorquens, Prot. Express.
Purif. 46, 316-320 (2006).
D. M. Colleluori, R. S. Reczknwski, F. A. Emig,
E. Cama, J. D. Cox, L. R. Scolnick, K. Compher, K. Jude, S. Han, R. E. Viola,
D. W. Christianson, and D. E. Ash, Probing the Role of the Hyper-Reactive
Histidine Residue of Arginase, Arch. Biochem. Biophys. 444,
15-26 (2005).
C. R. Faehnle, J. F. Ohren, and R. E. Viola, A
New Branch in the Family. Structure of Aspartate β-semialdehyde Dehydrogenase
from Methanococcus jannaschii, J. Molec. Biol. 353,
1055-1068 (2005).
C. R. Faehnle, J. Blanco, and R. E. Viola, Structural Basis
for Discrimination Between Oxyanion Substrates or Inhibitors in
Aspartate-β-semialdehyde Dehydrogenase, Acta.
Cryst.D60,
2320-2324 (2004).
J. Blanco,
R. A. Moore, C. R. Faehnle, and R. E. Viola,
Critical Catalytic Functional Groups in the Mechanism of
Aspartate-β-semialdehyde Dehydrogenase, Acta.
Cryst.D60,
1808-1815 (2004).
E. Cama, S. Pethe, J.-L.
Boucher, S. Han, F. A. Emig, D. E. Ash, R. E.
Viola, D. Mansuy, and D. W. Christianson,
Inhibitor Coordination Interactions in the Binuclear Manganese Cluster of Arginase, Biochemistry43, 8987-8999
(2004).
J. Blanco,
R. A. Moore, C. R. Faehnle, D. M. Coe, and R. E.
Viola, The Role of Substrate Binding Groups in the Mechanism of
Aspartate-β-semialdehyde Dehydrogenase, Acta
Cryst.D60,
1388-1395 (2004).
C. N. Madhavarao, J. R. Moffett, R. A. Moore, R. E. Viola,
M. A. A. Namboodiri, and D. M. Jacobowitz, Immunohistochemical
Localization of Aspartoacylase in the Rat Central Nervous System, J. Compar. Neurol.472,
318-329 (2004).
S. Han and
R. E. Viola, Splicing of Unnatural Amino Acids into Proteins:?A Peptide
Model Study, Prot. Pept. Lett.11, 107-114 (2004).
I. Elder,
S. Han, C. K. Tu, H. Steele, P. J. Laipis, R. E. Viola, and D. N. Silverman, Activation
of Carbonic Anhydrase II by Active-Site
Incorporation of Histidine Analogs, Arch. Biochem.
Biophys., 421,
283-289 (2004).
J. Blanco,
R. A. Moore, and R. E. Viola, Capture of an Intermediate in the Catalytic
Cycle of L-Aspartate-β-semialdehyde Dehydrogenase, Proc. Natl. Acad. Sci. USA100, 12613-12617 (2003).
S. Han, R.
A. Moore, and R. E. Viola, A Facile Synthesis of a Difluoromethylene
Analog of β-Aspartyl Phosphate as an Inhibitor of
L-Aspartate-β-Semialdehyde Dehydrogenases, SynLett.,
845-846 (2003).
R. A.
Moore, C. R. Faehnle, J. LeCoq,
and R. E. Viola, Purification and Preliminary Characterization of Brain
Aspartoacylase, Arch. Biochem. Biophys.413, 1-8 (2003).
J. Blanco,
R. A. Moore, V. Kabaleeswaram, and R. E. Viola,
A Structural Basis for the Mechanism of Aspartate-β-semialdehyde
Dehydrogenase from Vibrio Cholerae, Protein Sci
.12, 27-33 (2003).
R. A.
Moore, W. A. Bocik, and R. E. Viola, Expression
and Purification of L-Aspartate-β-semialdehyde dehydrogenase from
Infectious Microorganisms, Prot. Express. Purif .
25, 189-194 (2002).
C. L.
James and R. E. Viola, Production and Characterization of Bifunctional
Enzymes.?Substrate Channeling in the Aspartate Pathway, Biochemistry41, 3726-3731 (2002).
C. L.
James and R. E. Viola, Production and Characterization of Bifunctional
Enzymes.?Domain Swapping to Produce New Bifunctional
Enzymes in the Aspartate Pathway, Biochemistry41, 3720-3725
(2002).
S. Han, R.
A. Moore, and R. E. Viola, Synthesis and Evaluation of Alternative
Substrates for Arginase, Bioorg.
Chem.30, 81-94
(2002).
K. M.
Jude, S. K. Wright, C. K. Tu, D. N. Silverman,
R. E. Viola, and D. W. Christianson, Crystal Structure of
F65A/Y131C-methylimidazole Carbonic Anhydrase V
Reveals Architectural Features of an Engineered Proton Shuttle, Biochemistry41, 2485-2491 (2002).
J. Blanco
and R. E. Viola, Purification, crystallization and preliminary X-ray
analysis of aspartokinase III from Escherichia coli, Acta Cryst.D58, 352-354 (2002).
A. Hadfield, C. Shammes, G. Kryger, D. Ringe, G. Petsko, J. Ouyang, and R. E.
Viola, Active site analysis of the potential anti-microbial target
Aspartate semialdehyde dehydrogenase, Biochemistry40,
14475-14483 (2001).
S. Han and
R. E. Viola, A Spectrophotometric
Assay of Arginase, Anal. Biochem.295, 117-119 (2001).
J. K.
Bell, H. Yennawar, S. K. Wright, R. E. Viola,
and L. J. Banaszak, Structural Analysis of a
Malate Dehydrogenase with a Variable Active Site, J. Biol. Chem.276,
31156-31162 (2001).
S. K.
Wright and R. E. Viola, Alteration of the Specificity of Malate
Dehydrogenase by Chemical Modulation of an Active Site Arginine, J.
Biol. Chem.276, 31151-31155 (2001).
R. E.
Viola, The Central Enzymes of the Aspartate Pathway of Amino Acid
Biosynthesis, Acc. Chem. Res.34, 339-349 (2001).
Wright,
S.K.; Kish, M.M.; Viola, R.E., From Malate Dehydrogenase to Phenyllactate
Dehydrogenase: Incorporation of Unnatural Amino Acids to Generate an
Improved Enzyme-Catalyzed Activity, J. Biol. Chem. , in press
(2000).
Viola,
R.E. "L-Aspartase: New Tricks from an Old Enzyme", in Adv.
Enzymol., Mechanism of Enzyme Action, Vol. 74, Part B, (D. Purich,
ed.), 2000, pp. 295-341, Academic Press, San
Diego, CA.
Jayasekera,
M.M.K.; Viola, R.E., Recovery of Catalytic Activity from an Aggregated
Inactive Mutant of l-Aspartase, Biochem. Biophys. Res. Comm.264,
596-600 (1999).
Hadfield,
A.; Kryger, G.; Ouyang, J.; Petsko, G.A.; Ringe, D.; Viola, R.E.,
Structure of Aspartate semialdehyde Dehydrogenase from Escherichia
coli, a Key Enzyme in the Aspartate Family of Amino Acid Biosynthesis,
J. Molec. Biol.289, 991-1002(1999).
Viola,
R.E.; Wright, S.K. "New Enzymes from Old" in Enzymatic
Mechanisms, (P.A. Frey and D. Northrop, eds.), 1999, pp.116-130, IOS
Press, Amsterdam.
Earnhardt,
J.N.; Wright, S.K.; Qian, M.; Tu, C.K.; Laipis, P.J; Viola, R.E.;
Silverman, D.N., Introduction of Histidine Analogs Leads to Enhanced
Proton Transfer in Carbonic Anhydrase V., Arch. Biochem. Biophys.361,
264-270 (1999).
Scott,
M.E.; Viola, R.E., The Use of Fluoro- and Deoxy-Substrate Analogs to
Examine Binding Specificity and Catalysis in the Enzymes of the Sorbitol
Pathway, Carbohydrate Res.313, 247-253 (1999).
Wright,
S.K.; Viola, R.E., Evaluation of Methods for the Quantitation of Cysteines
in Proteins, Anal. Biochem.265, 8-14 (1998).
Jayasekera,
M.M.K.; Saribas, A.S.; Viola, R.E., Enhancement of Catalytic Activity by
Gene Truncation. Activation of L-Aspartase from Escherichia coli, Biochem.
Biophys. Res. Commun.238, 411-414 (1997).
Jayasekera,
M.M.K.; Shi, W.; Farber, G.K.; Viola, R.E., Evaluation of Functionally
Important Amino Acids in l-Aspartate Ammonia-Lyase from Escherichia
coli., Biochemistry, 36, 9145-9150 (1997).
Shi, W.;
Dunbar, J.; Jayasekera, M.M.K.; Viola, R.E.; Farber, G.K., The Structure
of l-Aspartate Ammonia-Lyase from Escherichia coli., Biochemistry,
36, 9136-9144 (1997).
Giorgianni,
F.; Beranova, S.; Wesdemiotis, C.; Viola, R.E., Mapping the
Mechanism-based Modification Sites in l-Aspartase from Escherichia
coli,Arch.Biochem. Biophys., 341, 329-336
(1996).
Huo, X.;
Viola, R.E., Substrate Specificity and Identification of Functional Groups
of Homoserine Kinase from Escherichia coli, Biochemistry,35,
16180-16185 (1996).
Schindler,
J.F.; Viola, R.E., Conversion of Cysteinyl Residues to Unnatural Amino
Acid Analogs. Examination in a Model System, J. Prot. Chem.15,
737-742, (1996).
Keng,
Y.-F.; Viola, R.E., Specificity of Aspartokinase III from Escherichia
coli and an Examination of Important Catalytic Residues , Arch.
Biochem. Biophys., 335, 73-81 (1996).
Huo, X.;
Viola, R.E., Functional Group Characterization of Homoserine Kinase Escherichia
coli,Arch.Biochem. Biophys.330, 373-379
(1996).
